Blog Latest articles in primer design category

Designing primers for PCR amplification

Designing primers is the first stage of many genetical and biological experiments. Primers are short oligonucleotide sequences that bind to the genomic sequence under PCR conditions. Well designed allow identification of genetic mutations or genomic fragments. The template, i.e. the identifiable sequence, can be any organism whose reference sequence is known and described in databases. Primers are commonly used in human genetic diagnostics, but they are equally often used to identify differences between species of different organisms.