16S analysis is widely used to identify microorganisms - bacteria, archaea - and to search for phylogenetic relationships between them. It involves the amplification and sequencing of the 16S gene, characterized by a high polymorphism between different species of these microorganisms. The 16S gene is divided into several regions: V1 to V9, whose amplification occurs due to universal primers. The amplification of two 16S regions (e.g. V3V4) is sufficient to identify most bacteria, however, the longer the fragment is amplified, the easier it is to distinguish microorganisms with a high degree of similarity. This is especially important for bacterial identification up to the species taxonomic level.