Mapping to reference sequences

Mapping is a technique that enables aligning reads to a reference genome sequence. A reference sequence may be the human genome hg18/hg19/hg38 or a genome of another organism (bacteria, fungi, viruses, etc.). The sequences can be obtained from the USCS or RefSeq (NCBI) databases.

We offer mapping of reads from next-generation sequencing FASTQ files to a specified reference genome sequence, on the basis of the Burrows-Wheeler algorithm. The result will be presented in a form of a report containing:

  • the analysis of the quality of aligned reads;
  • the total number of aligned reads;
  • the average sequencing coverage of reads over an entire experiment, a DNA fragment and single nucleotides;
  • percentage thresholds of nucleotide sequencing coverage;
  • a list of uncovered fragments;
  • and others;

We also offer our help during the search for an appropriate reference sequence in biological databases.