De novo genome analysis
De novo genome analysis is based on reading the genome sequence of an examined organism without the necessity of alignment with a known reference sequence. It involves the assembly of reads – obtained from the next-generation sequencing – using bioinformatic algorithms that merge analysed nucleotide sequences in order to reconstruct the entire genetic information contained in a genome. De novo genome analysis begins with the assembly of reads into sequences of contigs, which are subsequently assembled into scaffolds. During the final stage, scaffolds are assembled into the entire genome.
The analysis is widely used for small bacterial, fungal or viral genomes, especially when the examined organism is unknown or the aim is to find a new strain.
Our offer includes de novo reads assembly using available bioinformatic algorithms. The result is presented in the form of a report involving the statistical data of each specific stage of analysis.